Structural Evaluation, Equilibrium Studies, Binding Affinity and Antioxidant Properties; Antimicrobial and Cytotoxicity Assay of Novel Hydrazone with Nitrophenyl and Isonicotinoyl N-Oxide Moieties and Its 3d- Metal Complexes

DOI: 10.22607/IJACS.2025.1302006

Research Article

ABSTRACT

A novel isonicotinoyl N-oxide hydrazone and its cobalt, nickel, copper, and zinc metal complexes are characterized and their in vitro biological activity is explored. pH metric studies were performed in a 40% DMF-H2O medium to calculate the pKa values of the ligand under investigation to ascertain its basicity. HyerChem7.5 software was used to deduce contour maps of frontier orbitals HOMO and LUMO of title ligand to identify donor sites probability in forming metal complexes. The title hydrazone and its newly prepared metal complexes were evaluated for structural analysis by various spectroanalytical tools, namely, FT-IR, 1H-NMR, ESI-Mass, UV-Vis, TGA&DTA, SEM-EDX, and ESR. The complexes are assigned octahedral geometry with 1:2 (M[II]: Ligand) composition wherein two ligand moieties are coordinated through O- and N donor sites and with two coordinated water molecules. The synthesized compounds were investigated for DNA binding interactions with CT-DNA absorption and fluorescence techniques. The screening for antioxidant activity of all compounds was assessed with a DPPH radical scavenging assay. Antibacterial and antifungal studies conducted with all title compounds were helpful to evaluate their relative potential activities. The synthesized compounds tested against cell lines (MCF-7), for cytotoxic activity by adopting MTT assay is assertive to understand relatively their active property.

Key words:  Antimicrobial, Antioxidant, Contour maps, Cytotoxicity, DNA binding, Equilibrium studies.

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Indian Journal of Advances in Chemical Science,

Volume: 13, Issue : 2,  January 2025

ISSN No.: 2320-0898 (Print); 2320-0928 (Electronic)