Indian Journal of Advances in Chemical Science, Volume: 9, Issue :3, August 2021

ISSN No.: 2320-0898 (Print); 2320-0928 (Electronic)

DOI: 10.22607/IJACS.2021.903001

Research Article

Spectroscopic Investigation of Steroidal Benzothiazoles as DNA-Binding
Agents and their Role in Cytotoxicity

ABSTRACT

The new steroidal benzathiazole derivatives (4-6) were synthesized by the reaction of steroidal ketones (1-3) with 2-aminothiophenol in chloroform. After characterization by spectral and analytical data, the DNA interaction studies of one of the compounds were carried out by ultraviolet–visible, fluorescence spectroscopy, viscosity measurements, circular dichroism, molecular docking, and gel electrophoresis. The compound binds to DNA preferentially through electrostatic and hydrophobic interactions with Kb; 4.5 ×104 M−1 revealing its potential propensity toward computed tomography DNA. Gel electrophoresis demonstrated that compound 5 showed a strong interaction during the concentration-dependent cleavage activity with pBR322 DNA. The molecular docking study suggested the intercalation of compounds in the minor groove of DNA. During in vitro cytotoxicity (3-(4,5-dimethylthizao1-2-y1)- 2,5-diphenyltetrazolium bromide assay), compound (4-6) revealed potential toxicity against the different human cancer cells. Western blotting analysis clearly indicates that compound 5 causes apoptosis in HL-60 cancer cells. The results revealed that compound 5 has better prospectus to act as a cancer chemotherapeutic candidate, which warrants further in vivo anticancer investigations.

Key words:  Benzothiazole, DNA binding, 3-(4,5-Dimethylthizao1-2-y1)-2,5-diphenyltetrazolium bromide assay, Western blotting.

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