Abstract

We fabricated Co0.21Cu0.79O nanoparticles from co-precipitation followed by thermal decomposition of cobalt doped copper
creatinate hydrazinate ([Co0.21Cu0.79(Cre)2(N2H4)2]) inorganic precursor. The inorganic precursor was characterized through
energy dispersive X-ray spectroscopy (EDS), Inductively Coupled Plasma Atomic Emission Spectrophotometry (ICP-
AES), Fourier transform infrared (FT-IR), and thermogravimetry-differential thermal analyser analysis. To characterize the
composition, structural phase, chemical state, morphological, and textural properties of fabricated Co0.21Cu0.79O nanoparticles
techniques such as EDS, ICP-AES, X-ray diffraction, FT-IR, Raman, X-ray photoelectron spectroscopy, Scanning electron
microscopy, transmission electron microscope, and Brunauer–Emmett–Teller were used. The in vitro antioxidant activity of
Co0.21Cu0.79O nanoparticles was assessed by 2,2-diphenyl-1-picryl-hydrazyl-hydrate free radical assay. Furthermore, the in vitro
cytotoxic activity of Co0.21Cu0.79O nanoparticles was evaluated against human embryonic kidney 293 and HeLa cell lines using
3-[4,5-dimethylthiazol-2-yl]2,5-diphenyltetrazolium bromide assay.