Abstract

A simple, precise, and accurate method is developed and validated for the simultaneous evaluation of Omeprazole (OME),
Domperidone (DOM) in complex formulations (combined dosage form) using UV spectrophotometric methods. The method 1
developed is dual-wavelength method, measures the difference in the absorbance of the mixtures at wavelengths whereas single
drug has the same absorbance and vice versa. Mean centered ratio method which depends on the construction of calibration
by dividing spectra of the one compound with the other is reported as Method 2. The method 3 is derivative spectrum method,
depends on zero-crossing points of the derivative spectrum, which enables the construction of calibration for the two drugs in
the presence of second one. Simultaneous estimation of OME and DOM by the Q-absorption method and simultaneous equation
method is already reported. To maintain the identical conditions throughout the experiment, the two methods Simultaneous
equation method (Method 4) and the Q-Absorption method (Method 5) are repeated. All the five methods are tested for accuracy,
precision by six replicate experiments and recovery studies using known synthetic mixtures. The calibrations are used for the
analysis of two drugs in the tablet. The methods are validated in terms of ICH guidelines.