Abstract

A simple, sensitive, and accurate spectrophotometric method has been developed for the determination of riboflavin in pure and
pharmaceutical formulations. The method is based on the reaction of riboflavin with sodium nitroprusside in the presence of
hydroxylamine hydrochloride and sodium carbonate to give an intense greenish yellow colored species which absorb maximally
at 445 nm. Beer’s law is obeyed in the concentration range of 0.94–7.52 ppm with a coefficient of determination r2=0.9996.
Statistical analysis of this method exhibited relative standard deviation of 1.28% with the limit of detection 0.564 ppm and
limit of determination 1.690 ppm. The proposed method has been applied successfully for the determination of riboflavin in
pharmaceutical products and can be extended for the routine analysis in bulk drugs.