Abstract

An analytical approach has been developed and validated using liquid chromatography (LC)-mass Spectrometry (MS)/MS
for the simultaneous determination of isoquercitrin and astragalin from the TGT Primaage (plant [leaf] extract), following
the application of toxicology studies. The purpose of analytical method validation was established a sensitive data, which
was investigated for chronic toxicology studies evaluation. The method validations were implemented for determining the
individuality and concentration of the analytes, matrix match effects and provide an estimated analytical method validation. The
method validation was carried out by performing different parameters using reference standards and test substance solutions
of isoquercitrin and astragalin in the matrix and analyzed onto LC-MS/MS. This analytical validated method was successfully
applied to the actual samples of the toxicology studies for the dose formulation analysis of TGT Primaage (plant [leaf] extract)
samples which had the contents of isoquercitrin and astragalin.