Abstract

Two visible spectrophotometric methods were developed A and B for the determination of Tripolidine Hydrochloride in pure and
pharmaceutical formulations. Method A is based on the formation of co-ordination complex of tertiary amine of TPH (electron
donor) and the central metal of the cobalt thiocyanate (acceptor) and Method B is based on internal salt formation involving
aconitic anhydride (dehydration product of CiA) and the tertiary amine of TPH. The coloured products exhibit absorption λmax
at 623 nm and 546nm. Regression analysis of Beer-Lambert plots showed good correlation in the concentration ranges 8-48
μg/ml, correlation co-efficients are 0.9999(A), 0.9822(B);the Sandell’s sensitivities are 2.7705 x 10-3 , 1.5058 x 10-3 (1 mole
cm-1); molar absorptivity values are1.1364 x 105, 2.0908 x 105 (μg cm-2)for methods A,B respectively.The proposed methods are
applied to commercial available formulations and the results are statistically compared with those obtained by the UV reference
method and validated by recovery studies. The results are found satisfactory and reproducible. These methods are applied
successfully for the estimation of the TPH in the presence of other ingredients that are usually present in formulations. These
methods offer the advantages of rapidity, simplicity and sensitivity and low cost without the need for expensive instrumentation
and reagents.