Abstract

A potentially novel Aspergillus sp. as a producer of multienzymes is exploited in fungal fermentation on lignocellulosic
substrates. High-level production of extracellular cellulase (exoglucanase, endoglucanase, and β-glucosidase) and amylase has
been achieved. Three different types of “waste” solids such as sugarcane bagasse, sawdust, and tea residue have been used in
solid-state fermentation (SSF). Among the three different lignocellulosic substrates used in this study, sugarcane bagasse was
the excellent solid substrate for higher production of cellulase and amylase enzymes. Sugarcane bagasse supported remarkable
production of filter paperase (15.42 FPU/g of substrate), carboxymethyl cellulase (CMCase) (17.89 U/g of substrate) and
β-glucosidase (2.43 U/g of substrate), and amylase activity (15.12 U/g of substrate) in SSF. Significant secretion of protein
(45.75 mg/g of substrate) on sugarcane bagasse was noticed. Though, producing sustainable biofuels from lignocellulosic
substrates in a cost-effective manner, it is an important challenge in the commercialization of the production process.