Abstract

A novel isonicotinoyl N-oxide hydrazone and its cobalt, nickel, copper and zinc metal complexes are characterized and their in vitro biological activity is explored. pH metric studies were performed in 40 % DMF-H2O medium to calculate pKa values of ligand under investigation to ascertain its basicity. HyerChem7.5 software was used to deduce contour maps of frontier orbitals HOMO and LUMO of title ligand to identify donor sites probability in forming metal complexes. The title hydrazone and its newly prepared metal complexes were evaluated for structural analysis by various spectro analytical tools viz; FT-IR, 1H-NMR, ESI-Mass, UV-Vis, TGA&DTA, SEM-EDX and ESR. The complexes are assigned octahedral geometry with 1:2 [M(II): Ligand] composition wherein two ligand moieties are coordinated through O- and N donor sites and with two coordinated water molecules. The synthesized compounds were investigated for DNA binding interactions with CT-DNA absorption and fluorescence techniques. The screening for antioxidant activity of all compounds was assessed with DPPH radical scavenging assay. Antibacterial and antifungal studies conducted with all title compounds were helpful to evaluate their relative potential activities. The synthesized compounds tested against cell lines (MCF-7), for cytotoxic activity by adopting MTT assay is assertive to understand relatively their active property.