Abstract

A new series of N-(4-(substituted amino) thieno[2,3-d]pyrimidin-2-yl) thiophene/Furan-2-carboxamide (7 a-j)
derivatives were synthesized by a five-step procedure that afforded advantages of mild reaction conditions, simple
protocol and good yields. Several thieno[2,3-d]pyrimidines have been prepared from methyl 2-aminothiophene-
3-carboxylate (1). The structures of the final compounds were confirmed by infrared, nuclear magnetic resonance,
and electron impact mass spectrum. The final compounds were screened for their antibacterial activity against
Staphylococcus aureus and Bacillus subtilis from Gram-positive group of bacteria. Pseudomonas aeruginosa
and Escherichia coli from Gram-negative group of bacteria. Antifungal activity against Aspergillus niger and
Candida albicans. Antibacterial and antifungal activities were evaluated and compared with the standard drugs
such as amoxicillin and fluconazole. From antibacterial and antifungal activity screening results, it has been
observed that compounds 8j, 8i, 8h and 8g possess good activity.