Abstract

Incorporation of green chemistry principles to nanotechnology is one of the key concerns in nanoscience research.
There is growing need to develop environmentally benign metal nanoparticle fabrication progression that does
not use toxic chemicals in the synthetic conventions to avoid antagonistic effects in medical applications. In
this process using rapid, convenient and environment-friendly method for the fabrication of gold nanoparticles
(Prosopis juliflora [PJ]-GNPs) by reducing aurum chloride with the aqueous extract of PJ leaves. To elevate
the process parameters involved in the fabrication of PJ-GNPs by green method including extract concentration
Au+3 ion concentration and time intervals. The antibacterial activity is carried out by disc diffusion method.
The green synthesized PJ-GNPs are primarily confirmed by visual observations through color changing of the
reaction mixture from light yellow to ruby red. The surface plasmon resonance of PJ-GNPs is obtained at 520 nm,
and the effect of the phytochemicals present in PJ extract, including phenolic compounds, phytosterols, and
quinones on formation of stable PJ-GNPs are investigated by ultraviolet-visible spectroscopy, Fourier transform
infrared spectroscopy; the size of PJ-GNPs is confirmed by dynamic light scattering and transmission electron
microscopy. The morphology and shape of the PJ-GNPs are determined by scanning electron microscopy and
energy dispersive X-ray analysis. The antibacterial activity of PJ-GNPs is studied against pathogenic microbial
strains.