Abstract

Three simple, sensitive, and rapid spectrophotometric methods were developed for the estimation of bromhexine
(BRH) in pure and its commercial dosage forms. These methods were based on the formation of charge-transfer
reaction between the drug, an n-electron donor and π-acceptors, 2, 3-dichloro-5, 6-dicyano-p-benzoquinone,
chloranilic acid, and 1-chloro-2, 4-dinitrobenzene. The absorbance of the formed charge-transfer complexes was
measured and utilized for the determination of BRH in its pure and commercial dosage forms. The developed methods
were evaluated in terms of standard deviation, relative standard deviation, correlation coefficient, limit of detection,
and limit of quantitation. Molar absorptivity and Sandell’s sensitivity were calculated at the optimum experimental
conditions. The validity of the proposed methods was ascertained by recovery studies which indicated that the
present methods can be successfully applied for the determination of BRH in pure and commercial dosage forms.