Abstract

In this study, we present a novel method to achieve Candida antarctica B lipase immobilization on polyurethane
(PU) foam. The PU was an extremely simple support to be prepared and, the enzyme immobilization occurs
simultaneously with the polymerization, leading <5 min. Thus, the enzyme distribution was of 100% by irreversible
binding in the PU support matrix. The immobilization process provided a high yield of 250% in the enzymatic
catalytic activity compared to the free process. The immobilized presented recycles 35 of reuse, with the higher
residual activity of 80% in all cycles. In this way, this process provides an important technique associated with
low-cost of the support, simple procedure, and possibility of being employed as a catalyst in reactions for the food
industry.