Abstract

In the present study, four simple, rapid, and sensitive charge-transfer spectrophotometric methods were developed
for the determination of esmolol hydrochloride (ESM). 2,3-Dichloro-5, 6-dicyano-1, 4-benzoquinone, 1-chloro
2,4-dinitro benzene, 2,5-dichloro-3,6-dihydroxycyclohexa-2,5-diene-1,4-dione (chloranilic acid), and sodium
3,4-dioxo-3,4-dihydronaphthalene-1-sulfonate (Folin’s reagent) were utilized as reagents in method A, B, C,
and D, respectively. The developed charge- transfer complexes were measured at λmax of 460, 430, 410, and
490 nm, respectively. The developed methods were applied successfully for the determination of ESM in its pure
form as well as in its pharmaceutical formulations. The accuracy and reliability of the method were further
ascertained by performing recovery test via standard addition technique.