Abstract

A simple, rapid, and sensitive spectrophotometric method are described for the determination of hydrogen
peroxide using ammonium ferrous sulfate (FAS) and sulfosalicylic acid (SSA) are presented. This model is based
on the oxidation of FAS by H2O2 in acidic condition to form ferric ion, in aqueous medium sulfonic group of
SSA is completely ionized to give RH2- and RH2-. In acidic medium, this molecule binds to Fe (III) to form a
purple-colored FeSSA chelate compound, this has λmax at 510 nm. The optical density is directly proportional
to hydrogen peroxide concentration and obeys Beer’s law in the range 4.0-24.0 μM. The molar absorptivity,
Sandell’s sensitivity, detection limit, and quantification limit of the method were found to be 2.2×104 L mol−1 cm−1,
0. 2 μM/cm2, 0.073 μmol L−1, and 0.224 μmol L−1, respectively. The results of analysis of the proposed method are
compared favorably with those from a reference method. The method is successfully applied to water and plant
samples.