Abstract

Analytical chemistry has undergone a revolution thanks to ambient ionization mass spectrometry (AIMS), which makes it possible
to analyze samples quickly and directly in their natural state. The analytical community has led the continued development of
AIMS since its inception almost 20 years ago, spurred on by the plethora of benefits it offers in addition to conventional mass
spectrometry (MS). Since the development of desorption electrospray ionization MS, ambient MS techniques have become
more popular in a range of disciplines, including food science and health. Because it requires little sample preparation and
offers quick, direct analysis of samples in a range of physical states, AIMS is gaining popularity in the domain of metabolomics.
Consequently, attention in AIMS has rapidly grown among analytical communities around the world, and AIMS methodologies
are becoming more and more ingrained in regular laboratory procedures. This annual review describes the use of AIMS in
2022 with an emphasis on biomedical applications. Even though some new methods are being developed, AIMS research is
increasingly committed to improving the methods that already exist, particularly in terms of repeatability, quantification, and
functionality.