Abstract

A simple, accurate, and precise reverse-phase high-performance liquid chromatographic method was developed for the
estimation of fenoterol hydrobromide in pharmaceutical formulations. A mixture of 70 volumes of buffer solution was prepared
by adding 1 ml of triethylamine in 1000 ml of water adjust to pH = 5.0 with formic acid, and 30 volumes of acetonitrile at a
flow rate of 1.0 ml/min with UV detection at 276 nm. The concentration range was from 50% to 150%, the retention time was
6.163 min and the correlation coefficient of the analytical curve was 0.999. The limit of detection and limit quantification were
0.002 mg/mL and 0.006 mg/mL, respectively. Intra- and inter-day relative standard deviations were ≤2.0%. The methodology
accuracy showed the percentage between 98.61% and 101.11%. The described technique was found to be simple, rapid, precise,
accurate, and sensitive; the advantages over the other current methodologies are the low-cost and low-polluting conditions.
Owing to its simplicity and reliable results, this methodology is suitable to be used in the quality control of pharmaceutical drugs
containing fenoterol hydrobromide as an active component.